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EEM spectroscopy is effective for offline fluorescence analysis, but an additional testment of UV-vis absorption spectrum is required, complicating the instrument and hindering online monitoring. To address this, we propose the translation-corrected estimation by the Rayleigh scattering (TCERS) method, allowing absorbance estimation, IFE self-correction, and providing orthogonal spectroscopy information. This method streamlines fluorescence spectrometer design, enabling miniaturization and optimization for practical applications like environmental monitoring.

State:
Published
Link:
DOI: 10.1021/acs.analchem.3c01093